Related Experiment Video

Updated: Aug 16, 2026

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

Preferential expression of the transcription coactivator HTIF1alpha gene in acute myeloid leukemia and MDS-related

D Gandini1, C De Angeli, G Aguiari

  • 1Centro di Biotecnologie, Sezione di Studi Biochimici, Università degli Studi di Ferrara, Via L. Borsari 46, 44100 Ferrara, Italy.

Leukemia
|May 3, 2002
PubMed

Insights

HTIF1alpha, a gene linked to acute myeloid leukemias (AML), shows altered RNA expression in leukemic cells. Its expression varies by AML subtype and is high in myeloid cell lines, suggesting a role in myeloid differentiation.

Area of Science:

  • Molecular Biology
  • Hematology
  • Cancer Genetics

Background:

  • HTIF1alpha, a transcription coactivator, is encoded by a gene on chromosome 7q32-34, a region critical in acute myeloid leukemias (AML).
  • The gene's potential involvement in AML prompted investigation into its DNA structure and RNA expression in leukemic cells.

Purpose of the Study:

  • To investigate the DNA structure and RNA expression of HTIF1alpha in leukemic cells from AML patients.
  • To determine the correlation between HTIF1alpha expression and AML subtypes, myelodysplastic syndrome (MDS), and myeloid cell differentiation.

Main Methods:

  • Analysis of HTIF1alpha DNA structure and RNA expression in leukemic cells from 36 AML patients (28 de novo, 8 secondary to MDS).
  • Comparison of HTIF1alpha expression in different AML subtypes (M1-M5), MDS, and normal mononuclear cells.
  • Assessment of HTIF1alpha expression in myeloid cell lines (HL60, NB4, K562) during induced differentiation.

Main Results:

  • No abnormal HTIF1alpha DNA fragments were found; DNA loss was observed in patients with chromosome 7q32 deletions/translocations.
  • HTIF1alpha RNA was detected in acute myelocytic leukemic blasts but was nearly undetectable in normal mononuclear cells.
  • HTIF1alpha expression was higher in M1-M3 AML subtypes, low in M4-M5 AML, and significantly overexpressed in MDS-related AML (MDR-AML).

Conclusions:

  • HTIF1alpha expression is altered in AML and MDR-AML, suggesting its potential role in leukemogenesis.
  • HTIF1alpha expression levels vary across AML subtypes and are distinctly regulated during myeloid differentiation.
  • HTIF1alpha may play a significant role in myeloid differentiation processes within hematopoietic lineages.

More Related Videos

Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia06:33

Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia

Published on: November 10, 2023

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...