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Soluble LDL-immune complexes in type 2 diabetes and vascular disease
1Vuk Vrhovac University Clinic for Diabetes, Dugi dol 4A, 10000 Zagreb, Croatia.zturk@idb.hr
Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|May 3, 2002
Summary
Elevated levels of LDL-ICs, linked to oxidized LDL, are a significant risk factor in type 2 diabetes and the general population. These immune complexes, rather than oxLDL antibodies alone, likely contribute more to atherosclerosis development.
Area of Science:
- Cardiovascular Research
- Immunology
- Metabolic Disorders
Background:
- Oxidative modification of LDL impacts its clearance and can induce cytotoxic and immunogenic responses.
- LDL containing immune complexes (LDL-ICs) are implicated in vascular disease pathology.
Purpose of the Study:
- To analyze markers of LDL oxidation, specifically LDL-ICs, in type 2 diabetes patients with micro- and macrovascular complications.
- To investigate the relationship between LDL-ICs, advanced glycated end-products (AGEs), and cardiovascular risk factors like triglycerides.
Main Methods:
- Study included diabetic patients with coronary artery disease (DM + CAD), non-diabetics with CAD, controls, and diabetics with or without nephropathy.
- Quantified oxLDL antibodies, AGEs via ELISA, and LDL-IC apo B content using PEG precipitation.
Main Results:
- Apo B content in LDL-ICs was significantly higher in patients with CAD and diabetes compared to controls.
- LDL-IC levels were elevated in diabetic patients with or without microangiopathy versus healthy volunteers.
- A positive correlation was found between AGE content and LDL-ICs; an inverse correlation existed between triglycerides and LDL-ICs in diabetic and CAD patients.
Conclusions:
- Increased circulating LDL-ICs represent a risk factor for the general population, including individuals with diabetes.
- LDL-ICs likely play a more significant role in atherosclerosis development than oxLDL antibodies alone.