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Global analysis of gene expression patterns in developing mouse neocortex using serial analysis of gene expression.
J M Gunnersen1, C Augustine, V Spirkoska
1Howard Florey Institute, University of Melbourne, Royal Parade, Parkville 3010, Victoria, Australia.
Molecular and Cellular Neurosciences
|May 4, 2002
Summary
This study used serial analysis of gene expression (SAGE) to compare gene expression in developing mouse neocortex before and after birth. Researchers identified 321 differentially expressed transcripts, including known and novel genes involved in neocortical development.
Area of Science:
- Developmental Neuroscience
- Molecular Biology
- Genomics
Background:
- The developing mammalian neocortex undergoes complex molecular changes.
- Understanding gene expression dynamics is crucial for elucidating developmental processes.
Purpose of the Study:
- To create molecular inventories of the developing mouse neocortex.
- To identify differentially expressed genes during the transition from embryonic to postnatal development.
Main Methods:
- Serial analysis of gene expression (SAGE) was employed to profile gene expression.
- Libraries were constructed from embryonic day 15 and postnatal day 1 mouse neocortex.
- Over 40,000 SAGE tags were analyzed.
Main Results:
- A total of 321 transcripts showed differential expression (P < 0.05) between the two developmental stages.
- Differential expression was validated using Northern blotting and in situ hybridization.
- Identified genes included known developmental regulators and novel candidates.
Conclusions:
- Gene expression profiles reveal significant molecular shifts during neocortical development.
- This study provides a valuable resource for understanding neocortical development and identifying new molecular players.