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Related Experiment Videos

New trends in gamete's cryopreservation.

Amir Arav1, Saar Yavin, Yoel Zeron

  • 1Institute of Animal Science, Agricultural Research Organization, The Volcani Center, PO Box 6, 50250, Bet-Dagan, Israel. arav@agri.huji.ac.il

Molecular and Cellular Endocrinology
|May 4, 2002
PubMed
Summary

New cryopreservation techniques enhance sperm, oocyte, and embryo survival. Advanced freezing and vitrification methods ensure high post-thaw motility and successful embryo development, improving assisted reproduction outcomes.

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Area of Science:

  • Reproductive Biology
  • Cryobiology
  • Biotechnology

Background:

  • Cryopreservation is crucial for preserving gametes and embryos.
  • Existing methods face challenges in optimizing cell survival rates.
  • Advancements are needed to improve the efficiency and success of assisted reproductive technologies.

Purpose of the Study:

  • To develop and evaluate novel cryopreservation techniques for sperm, oocytes, and embryos.
  • To enhance cell viability and developmental potential post-thaw.
  • To optimize freezing and vitrification processes for improved reproductive outcomes.

Main Methods:

  • Developed 'Multi-Thermal-Gradient' (MTG) freezing for sperm, controlling ice crystal propagation.
  • Utilized a 'Minimum Drop Size' vitrification technique with rapid cooling in super-cooled liquid nitrogen (-210°C).

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  • Applied these methods to various species including bovine, human, and other livestock.

    • Main Results:

    • Achieved 70-100% post-thaw motility for frozen sperm from multiple species.
    • Demonstrated high survival rates for bovine oocytes and embryos post-vitrification.
    • Vitrified MII oocytes showed 50% cleavage and 20% blastocyst rates.
    • Produced a healthy calf from vitrified in-vitro produced (IVP) bovine embryos.

    Conclusions:

    • Novel MTG freezing and Minimum Drop Size vitrification significantly improve cryopreservation of gametes and embryos.
    • These advanced techniques offer high viability and developmental potential, crucial for assisted reproduction.
    • Successful application across species, including the birth of a calf, validates the efficacy of the developed methods.