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Effects of histamine on phosphoinositide metabolism in chick cerebral cortex
J B Zawilsk1, A Woldan-Tambor, K Kubal
1Department of Biogenic Amines, Polish Academy of Sciences, Lódź.
Abstract:
Effects of histamine (HA) and agonists of HA receptors on phosphoinositide metabolism in chick cerebral cortex have been studied using two approaches - measurement of inositol 1,4,5-trisphosphate (IP3) level by a specific and sensitive IP3 receptor radioassay, and analysis of [3H]inositol phosphates accumulation in cortical slices prelabeled with myo-[3H]inositol. HA concentration-dependently elevated IP3 levels in slices of chick cerebral cortex. The effect of HA was mimicked by 2-methylHA, a selective agonist of H1-HA receptors, and blocked by mepyramine, an H1 receptor antagonist. 4-MethylHA and Ralpha-methylHA, selective agonists of H2- and H3-HA receptors, respectively, did not affect IP3 level in the chick cerebrum. In cerebral cortical slices prelabeled with myo-[3H]inositol, 2-methylHA significantly stimulated [3H]inositol phosphates accumulation, whereas HA only slightly and non-significantly increased phosphoinositide metabolism. It is suggested that phospholipase C-coupled H1-HA receptors are present in the chick cerebral cortex, yet their number seems to be a small one.
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