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Updated: Oct 1, 2026

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri
Published on: April 24, 2018
Lesions induced by 2,4-D and chlorpyrifos in tench (Tinca tinca L.): implication in toxicity studies
1Histology and Pathological Anatomy Unit, Facultad de Veterinaria, Uex, Cáceres, Spain. luih@unex.es
Abstract:
This study was undertaken to demonstrate the toxicity of two pesticides, chlorpyrifos (O,O-diethyl 0-3,5,6-trichloro-2-pyridyl phosphorothioate) and 2,4-D (Dichlorophenoxyacetic acid) to tench (Tinca tinca Linnaeus). Pathological samples of damaged tench kidney showing lesions were taken for statistical analysis in order to quantify different parameters. Analysis revealed differences in the action and/or action time of the two pesticides. These differences were more acute in the case of 2,4-D thereby indicating a greater toxicity. These differences, expressed by mathematical formulae of numerical variables, would enable, a priori, the identification of the responsible pesticide and the time of acting in new natural cases of poisonings with these substances.

