Related Experiment Video
Updated: Jun 13, 2026

Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac
Published on: March 15, 2018
Fast and efficient separation of cytokinins from auxin and abscisic acid and their purification using mixed-mode
Petre Ivanov Dobrev1, Miroslav Kamínek
1Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha.
Abstract:
A method for separation of cytokinins from auxin and abscisic acid, which allows further separation of cytokinin ribotides from cytokinin bases, ribosides and glucosides and their purification on a single Oasis MCX column was developed. Due to the mixed reversed-phase and cation-exchange mode of the Oasis MCX sorbent the cationic cytokinin bases, ribosides and glucosides as well as the anionic auxin, abscisic acid and cytokinin ribotides are retained and can be sequentially eluted by solvents containing different concentrations of methanol and ammonium hydroxide. Characteristics of the method are high recoveries of analyzed phytohormones and their sufficient purity for quantification by HPLC-ELISA (RIA) or HPLC-MS.
More Related Videos
09:57Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
Published on: June 14, 2020
08:56Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
Published on: November 22, 2024
Related Concept Videos
Overview Of Cell Separation And Isolation
Ion-Exchange Chromatography
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Affinity Chromatography
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...