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Porcine ileitis model induced by TNBS-ethanol instillation
A M Merritt1, C D Buergelt, L C Sanchez
1Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610, USA.
Digestive Diseases and Sciences
|May 7, 2002
Summary
Trinitrobenzene sulfonic acid-ethanol (TNBS-EtOH) successfully induced severe ileitis in pigs when confined to the distal ileum. This model shows promise for studying Th-1 mediated inflammation in ileal disease.
Area of Science:
- Gastroenterology
- Veterinary Pathology
- Immunology
Background:
- Inflammatory bowel disease (IBD) research requires reliable animal models.
- Trinitrobenzene sulfonic acid (TNBS) is used to induce colitis, but its efficacy in inducing ileitis is less established.
- Understanding the immune mechanisms (Th-1 vs. Th-2) in ileitis is crucial for developing targeted therapies.
Purpose of the Study:
- To evaluate the efficacy of a trinitrobenzene sulfonic acid-ethanol (TNBS-EtOH) mixture in inducing ileitis in young pigs.
- To establish a porcine model for studying Th-1 mediated ileal inflammation.
Main Methods:
- Nine young pigs underwent laparotomy for access to the distal ileum.
- Trinitrobenzene sulfonic acid-ethanol (TNBS-EtOH) was instilled via hypodermic needle into the distal ileum.
- Instillate was either not confined or confined to a 10-cm segment for 10-15 minutes.
Main Results:
- Pigs with unconfined instillate showed no ileal lesions at two weeks.
- Pigs with confined TNBS-EtOH instillation developed severe gross and histologic ileitis within one week.
- Histopathology indicated a Th-1 mediated inflammatory response.
Conclusions:
- Confining TNBS-EtOH instillation to a specific segment of the distal ileum is essential for inducing ileitis in pigs.
- This method provides a viable porcine model for studying ileitis, particularly Th-1 mediated inflammation.
- The porcine model may aid in understanding and treating human inflammatory bowel diseases.