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Gap junction intercellular communication during lymphocyte transendothelial migration.

Ernesto Oviedo-Orta1, Rachel J Errington, W Howard Evans

  • 1Department of Medical Biochemistry, University of Wales College of Medicine, HeathPark Cardiff, CF14 4XN, UK. e.oviedo-orta@bristol.ac.uk

Cell Biology International
|May 7, 2002
PubMed
Summary
This summary is machine-generated.

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Lymphocyte migration involves cell-cell communication. While gap junctions facilitate intercellular dye transfer between lymphocytes and endothelial cells, they do not significantly impact lymphocyte transmigration itself.

Area of Science:

  • Immunology
  • Cell Biology
  • Endothelial Biology

Background:

  • Lymphocyte migration across endothelium is crucial for immune responses.
  • This process involves cell-cell interactions and heterotypic signaling.
  • Lymphocytes and endothelial cells express connexins, forming gap junctions.

Purpose of the Study:

  • To investigate the role of gap junctions in heterotypic signaling during lymphocyte transendothelial migration.
  • To determine if connexin-mediated communication influences lymphocyte migration.

Main Methods:

  • Flow cytometry (FACS) analysis using calcein dye transfer.
  • Inhibition studies with connexin mimetic peptides.
  • Two-photon laser scanning microscopy.

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Main Results:

  • Calcein dye transfer from endothelial cells to lymphocytes confirmed gap junction permeability.
  • Connexin mimetic peptides inhibited intercellular dye transfer, validating gap junction involvement.
  • Despite inhibiting gap junction communication, these peptides minimally affected lymphocyte transmigration.

Conclusions:

  • Gap junctions mediate intercellular communication between lymphocytes and endothelial cells during migration.
  • However, this gap junctional communication is not essential for the physical process of lymphocyte transmigration.