Basic studies on a labeled anti-mucin antibody detectable by infrared-fluorescence endoscopy

Area of Science:

• Biomedical Imaging
• Oncology
• Molecular Diagnostics

Background:

• Development of indocyanine green (ICG)-sulfo-OSu, an infrared-excitable fluorescent dye for antibody conjugation.
• Investigation of antisulfomucin and anti-MUC1 antibody staining patterns in gastrointestinal cancers.
• Evaluation of the dye as a fluorescent label for cancer-specific antibodies in diagnostic imaging.

Purpose of the Study:

• To clarify staining patterns of antisulfomucin and anti-MUC1 antibodies in gastrointestinal cancer.
• To assess the potential of ICG-sulfo-OSu-labeled antibodies for microcancer diagnosis via infrared fluorescence endoscopy.

Main Methods:

• Immunohistological staining of gastrointestinal cancer tissue samples (esophageal, gastric, colorectal) using anti-sulfomucin and anti-MUC1 antibodies.
• Application of ICG-sulfo-OSu-labeled antibodies to sections with high staining intensity.
• Evaluation of infrared fluorescence imaging for visualization of stained cancer cells.

Main Results:

• Antibody staining patterns varied by organ, histology, and cancer depth, but generally showed good retention on the mucosal surface of cancer tissues.
• High-quality images of cancer cells were obtained using ICG-sulfo-OSu-labeled anti-MUC1 antibody.
• Nearly specific infrared fluorescence was observed in cancer tissues with the labeled anti-MUC1 antibody.

Conclusions:

• The anti-MUC1 antibody effectively stains gastrointestinal cancer cells.
• ICG-sulfo-OSu-labeled anti-MUC1 antibody demonstrates potential utility for cancer screening.
• Infrared fluorescence endoscopy with labeled anti-MUC1 antibody is a promising diagnostic approach for gastrointestinal cancers.