Quantitative determination of 1 -hydroxypyrene in bovine urine samples using high-performance liquid chromatography...

An analytical method was developed to determine quantitatively 1-hydroxypyrene (OHP) in bovine urine samples. The procedure includes an enzymatic hydrolysis to cleave the conjugated metabolite, an enrichment step using solid phase extraction with a non-polar rinse step and elution with dichloromethane. A final clean-up on silicagel was performed before high-performance liquid chromatography (HPLC) analysis and fluorescence detection. Alternatively, HPLC and electrospray ionization in the negative ion mode applying selective ion monitoring acquisition revealed to be a highly sensitive detection method allowing the quantitation of low pg of OHP in the urine samples. The method was successfully applied to the determination of OHP in bovine urine samples from animals living in urban and rural areas. Urine concentrations of OHP were significantly higher (median 8.6 microg l(-1)) of bovines living close to a highway.