Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sin recombinase from Staphylococcus aureus: synaptic complex architecture and transposon targeting.

Sally-J Rowland1, W Marshall Stark, Martin R Boocock

  • 1Institute of Biomedical and Life Sciences, University of Glasgow, Anderson College, UK. sr2q@udcf.gla.ac.uk

Molecular Microbiology
|May 8, 2002
PubMed
Summary

The Sin recombinase from Staphylococcus aureus forms a unique DNA complex for strand exchange. Architectural protein Hbsu aids DNA deformation, similar to other resolvases, in this DNA recombination process.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct observation of subunit rotation during DNA strand exchange by serine recombinases.

Nature communications·2024
Same author

Variable orthogonality of serine integrase interactions within the ϕC31 family.

Scientific reports·2024
Same author

Variable orthogonality of RDF - large serine integrase interactions within the ϕC31 family.

bioRxiv : the preprint server for biology·2024
Same author

High fidelity one-pot DNA assembly using orthogonal serine integrases.

Biotechnology journal·2022
Same author

Structural basis for topological regulation of Tn3 resolvase.

Nucleic acids research·2022
Same author

The protein-protein interactions required for assembly of the Tn3 resolution synapse.

Molecular microbiology·2021

Area of Science:

  • Molecular Biology
  • Genetics
  • Structural Biology

Background:

  • Sin recombinase from Staphylococcus aureus mediates DNA recombination.
  • Recombination involves a specific DNA site, resH, and architectural protein Hbsu.
  • Understanding the DNA-protein complex is crucial for DNA repair and genetic engineering.

Purpose of the Study:

  • To elucidate the structural organization of the Sin recombinase-DNA complex.
  • To understand the role of Hbsu in DNA recombination.
  • To compare the mechanism of Sin recombinase with other known site-specific recombinases.

Main Methods:

  • Structural analysis of DNA-protein interactions.
  • Biochemical assays to study strand exchange.
  • Comparative analysis of recombination mechanisms.

Related Experiment Videos

Main Results:

  • Sin recombinase binds to two distinct sites within the resH recombination site.
  • Inverted and tandem motifs within resH are recognized by different Sin dimers.
  • Hbsu binds a central site, inducing DNA deformation that aligns with Tn552 integration targets.
  • Sin recombinase exhibits similar topological selectivity to Tn3 and gammadelta resolvases.

Conclusions:

  • A model for the recombination synapse involving four Sin dimers and Hbsu is proposed.
  • Hbsu plays a critical architectural role, analogous to resolvase dimers in other systems.
  • The findings provide insights into the mechanism of site-specific DNA recombination and its regulation.