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Enhanced monocyte tissue factor expression in hepatosplenic schistosomiasis
1Haematology Department, Theodore Bilharz Research Institute, Giza, Egypt. aliaaamer@yahoo.com
Abstract:
Monocyte tissue factor expression was evaluated in 67 patients with hepatosplenic Schistosomiasis. They were classified as Child A (n = 15), Child B (n = 15), Child C (n = 12) and Bleeders (n = 10), in addition to 15 healthy controls. Mononuclear cells were cultured in vitro with and without lipopolysaccharide (LPS) to assess monocyte tissue factor (TF) antigen (Ag) and activity (Act) in cell lysate, in addition to measurement of prothrombin fragment 1 + 2 (F1 + 2) as a marker of in vivo thrombin generation. A significant increase in monocyte TF Ag and TF Act was noted in all stages of the disease compared with the control group, with marked accentuation during an acute attack of variceal bleeding. This enhanced monocyte expression was noted before the addition of LPS and became more obvious with addition of LPS. An increasing level of F1 + 2 was similarly noted. These findings constitute further evidence for an existing prothrombotic state in hepatosplenic Schistosomiasis, and also that monocytes are closely implicated in the haemostatic diathesis characterizing the disease.
Insights
Patients with hepatosplenic Schistosomiasis show increased monocyte tissue factor (TF) expression, indicating a prothrombotic state. This heightened TF expression is linked to thrombin generation and disease severity, especially during bleeding episodes.
Area of Science:
- Hematology
- Infectious Diseases
- Pathophysiology
Background:
- Hepatosplenic Schistosomiasis is a chronic parasitic disease.
- Hemostatic abnormalities and a prothrombotic state are suspected in this condition.
Purpose of the Study:
- To investigate monocyte tissue factor (TF) expression and activity in patients with hepatosplenic Schistosomiasis.
- To assess the relationship between TF expression, thrombin generation, and disease severity.
Main Methods:
- Monocyte tissue factor (TF) antigen and activity were measured in 67 patients and 15 controls.
- In vitro stimulation with lipopolysaccharide (LPS) was used.
- Prothrombin fragment 1 + 2 (F1 + 2) was measured as a marker of thrombin generation.
Main Results:
- All patient groups exhibited significantly higher monocyte TF antigen and activity compared to controls.
- TF expression was most pronounced during acute variceal bleeding.
- Elevated F1 + 2 levels indicated increased in vivo thrombin generation across disease stages.
Conclusions:
- Monocytes play a significant role in the prothrombotic state associated with hepatosplenic Schistosomiasis.
- Enhanced monocyte TF expression contributes to the hemostatic diathesis observed in the disease.
