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Related Experiment Videos

Preventive effects of Impatiens balsamina on the hen egg-white lysozyme (HEL)-induced decrease in blood flow.

Kyoko Ishiguro1, Yukiko Ohira, Hisae Oku

  • 1School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Hyogo, Japan. ishiguro@mwu.mukogawa-u.ac.jp

Biological & Pharmaceutical Bulletin
|May 9, 2002
PubMed
Summary

Monitoring blood flow in mice offers a new way to study anaphylaxis. This method identified anti-anaphylactic compounds in Impatiens balsamina L. petals, showing potential for natural product research.

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Area of Science:

  • Pharmacology
  • Immunology
  • Natural Product Chemistry

Background:

  • Anaphylaxis is a severe allergic reaction requiring reliable monitoring methods.
  • Current methods for studying anaphylaxis in mice, like blood pressure monitoring, have limitations.
  • Investigating natural products for anti-anaphylactic properties is an ongoing area of research.

Purpose of the Study:

  • To establish blood flow monitoring as a reliable method for studying anaphylaxis in unanesthetized mice.
  • To evaluate the anti-anaphylactic effects of an extract from Impatiens balsamina L. petals.
  • To identify key mediators involved in the anaphylactic response studied.

Main Methods:

  • Utilized a Doppler blood flow meter to monitor changes in blood flow in unanesthetized mice during anaphylaxis.

Related Experiment Videos

  • Induced Hen egg-white lysozyme (HEL)-specific anaphylaxis.
  • Assessed the inhibitory effects of Impatiens balsamina L. petal extract (IB) and known anti-allergic agents on blood flow decrease.
  • Investigated the roles of platelet-activating factor (PAF), serotonin, and histamine in the observed blood flow changes.

    • Main Results:

    • Blood flow monitoring effectively estimated anaphylactic responses in mice.
    • The 35% ethanol extract (IB) of Impatiens balsamina L. petals demonstrated significant anti-anaphylactic effects.
    • Kaempferol 3-rutinoside and lawsone, isolated from IB, were identified as active compounds inhibiting blood flow decrease.
    • Platelet-activating factor (PAF) and serotonin were found to contribute to the decrease in blood flow during anaphylaxis, while histamine did not.

    Conclusions:

    • Blood flow monitoring is a viable and effective alternative for studying anaphylaxis and screening for anti-anaphylactic substances.
    • Impatiens balsamina L. petals contain compounds with significant anti-anaphylactic activity.
    • PAF and serotonin play a role in the anaphylactic blood flow response, offering targets for therapeutic intervention.