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Updated: Jul 31, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
Structure of the O-polysaccharide from the lipopolysaccharide from Vibrio cholerae O6
Niklas Bergström1, G Balakrish Nair, Andrej Weintraub
1Analytical Unit, Clinical Research Centre, Karolinska Institute, Huddinge University Hospital, Novum, SE-141 86, Sweden.
Abstract:
The O-polysaccharide from Vibrio cholerae O6 was isolated from the LPS by mild-acid hydrolysis and has been investigated by sugar and methylation analysis and NMR spectroscopy. The polysaccharide was also depolymerized with aqueous hydrofluoric acid to give the repeating unit and multiples thereof. The O-polysaccharide had the following tetrasaccharide repeating unit. Two O-acetyl groups are present, one of them making the GlcNAc residue fully substituted and the steric crowding considerable at the branching residue.
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