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[Colonisation studies using Campylobacter jejuni in chicks].

F Schulze1, W Erler

  • 1Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin Fachbereich 4 Bakterielle Tierseuchen und Bekämpfung von Zoonosen, Jena. f.schulze@bgvv.de

DTW. Deutsche Tierarztliche Wochenschrift
|May 10, 2002
PubMed
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This study investigated Campylobacter jejuni colonization in chicks, finding significant variation among strains. Some strains caused strong, long-lasting colonization, while others were eliminated or caused delayed infection, with IgG levels increasing post-colonization.

Area of Science:

  • Veterinary Microbiology
  • Immunology
  • Poultry Science

Background:

  • Campylobacter jejuni is a significant foodborne pathogen.
  • Understanding its colonization dynamics in poultry is crucial for food safety.
  • Specific pathogen-free (SPF) chicks are a common model for avian disease studies.

Purpose of the Study:

  • To evaluate the colonizing capability of different Campylobacter jejuni strains in White Leghorn chicks.
  • To investigate the development of specific antibodies (IgG, IgA, IgM) in response to C. jejuni colonization.
  • To determine the influence of chick age on C. jejuni colonization and antibody response.

Main Methods:

  • Experimentally colonized SPF chicks with 11 different C. jejuni strains via oesophageal gavage at 2 or 8 days of age.

Related Experiment Videos

  • Collected blood, liver, and feces samples at multiple time points post-inoculation (7-56 days).
  • Measured Campylobacter-specific IgG, IgA, and IgM antibodies using indirect ELISA; reisolated C. jejuni to assess colonization.

    • Main Results:

    • Significant variation in colonization capability among C. jejuni strains was observed; 5 strains established strong, long-lasting colonization, 2 caused weak/delayed colonization, and 4 were not reisolated.
    • Chicks inoculated at 8 days of age were colonized faster than those inoculated at 2 days, potentially due to higher maternal antibodies in younger chicks.
    • C. jejuni colonization led to a marked increase in serum IgG levels, correlating positively with pathogen counts in the ceca, while IgM and IgA levels remained stable.

    Conclusions:

    • The colonizing ability of C. jejuni strains varies considerably, impacting their persistence in poultry.
    • Chick age influences the speed of C. jejuni colonization, with younger chicks showing a delayed response possibly linked to maternal immunity.
    • Serum IgG increase serves as a potential indicator of C. jejuni colonization intensity in chicks.