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Related Experiment Videos

Induced sputum: time from expectoration to processing.

A Efthimiadis1, L Jayaram, S Weston

  • 1Asthma Research Group, Firestone Institute for Respiratory Health, St Joseph's Healthcare and McMaster University, Hamilton, Ontario, Canada.

The European Respiratory Journal
|May 10, 2002
PubMed
Summary
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Induced sputum processing for asthma research can be delayed up to 9 hours for cell counts and 18 hours for inflammatory markers when stored at 4°C, improving sample accessibility.

Area of Science:

  • Pulmonary Medicine
  • Clinical Immunology
  • Respiratory Research

Background:

  • Induced sputum analysis is crucial for assessing airway inflammation in asthma.
  • A perceived limitation is the requirement for immediate sample processing within 2 hours.
  • Delayed processing may affect the reliability of inflammatory markers and cell counts.

Purpose of the Study:

  • To investigate the impact of delayed processing on induced sputum analysis in asthma.
  • To determine optimal storage durations for cell counts and fluid-phase inflammatory markers.

Main Methods:

  • Induced sputum samples from 30 asthma patients were collected and stored at 4°C.
  • Samples were processed at 2 hours (baseline), 9 hours, and 18 hours.
  • Analysis included cell counts, cell viability, eosinophil cationic protein (ECP), interleukin-8 (IL-8), and fibrinogen.

Related Experiment Videos

Main Results:

  • Processing at 9 hours showed excellent repeatability (ICC 0.88-0.99) for all measured parameters compared to 2 hours.
  • By 18 hours, cell viability decreased significantly (65.0% to 43.0%), and repeatability for cell counts and some markers was reduced.
  • Fluid-phase markers (ECP, IL-8, fibrinogen) maintained good repeatability (ICC >0.75) up to 18 hours.

Conclusions:

  • Induced sputum cell counts can be reliably analyzed up to 9 hours post-collection when stored at 4°C.
  • Fluid-phase inflammatory markers (ECP, IL-8, fibrinogen) are stable for up to 18 hours under the same conditions.
  • Delayed processing is feasible, enhancing the practicality of induced sputum analysis in asthma research.