Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multi-charged oligonucleotide ion formation in sonic spray ionization.

Min Huang1, Atsumu Hirabayashi

  • 1Life Science Group, Hitachi Ltd., Kawagoe, Saitama, Japan.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
|May 10, 2002
PubMed
Summary

Oligonucleotide ion formation in mass spectrometry is complex. This study reveals how matrix and operating parameters influence ion charge states, with Tris enhancing higher charges and acetic acid favoring lower charges.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Associations of HSD11B1 polymorphisms with tacrolimus concentrations in Chinese renal transplant recipients with prednisone combined therapy.

Drug metabolism and disposition: the biological fate of chemicals·2015
Same author

Heparin inhibits the inflammatory response induced by LPS and HMGB1 by blocking the binding of HMGB1 to the surface of macrophages.

Cytokine·2015
Same author

Randomized clinical trial of brewed green and black tea in men with prostate cancer prior to prostatectomy.

The Prostate·2014
Same author

Hepatic angiomyolipomas: ultrasonic characteristics of 25 patients from a single center.

Ultrasound in medicine & biology·2014
Same author

Expression levels of microRNA-199 and hypoxia-inducible factor-1 alpha in brain tissue of patients with intractable epilepsy.

The International journal of neuroscience·2014
Same author

[Prevalence study on pneumoconiosis in 1367 dust exposed workers].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2014

Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • Oligonucleotides form negative ions in mass spectrometry, but ion formation is complex and sensitive to sample conditions.
  • Understanding ion formation is crucial for accurate oligonucleotide analysis.

Purpose of the Study:

  • To investigate oligonucleotide ion formation using ion trap mass spectrometry with sonic spray ionization.
  • To determine the effects of matrix components and operating parameters on ion production and charge states.

Main Methods:

  • Utilized an ion trap mass spectrometer coupled with a sonic spray ionization (SSI) source.
  • Analyzed a 20-base oligonucleotide, varying parameters like high voltage and flow rate.
  • Investigated the impact of additives such as Tris and acetic acid on ion formation.

Related Experiment Videos

Main Results:

  • The most abundant ion observed had approximately one charge per three bases.
  • Signal intensity and mass spectrum patterns were highly sensitive to matrix composition and operating parameters.
  • Tris addition enhanced signal intensity and promoted higher charge states, while acetic acid favored lower charge states.

Conclusions:

  • Oligonucleotide ion formation is influenced by various factors, leading to sensitivity in mass spectrometry.
  • Matrix composition and operating parameters significantly affect ion states and abundance.
  • Additives like Tris and acetic acid can be used to modulate oligonucleotide ion formation and charge states.