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Related Experiment Videos

Leukocyte migration test in a modified agarose medium.

R Koonakosit, B Petchclai

    American Journal of Clinical Pathology
    |October 1, 1975
    PubMed
    Summary
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    This study optimized the leukocyte migration test medium with HEPES, improving its correlation with intradermal tests for tuberculosis diagnosis. The enhanced medium allows testing in standard incubators, simplifying the procedure.

    Area of Science:

    • Immunology
    • Cellular Biology

    Background:

    • The leukocyte migration test is a valuable diagnostic tool.
    • Standardization of cell-based assays is crucial for reliable results.
    • Previous leukocyte migration test protocols required specialized incubation conditions.

    Purpose of the Study:

    • To optimize the medium for the leukocyte migration test.
    • To enhance the correlation of the leukocyte migration test with established diagnostic methods.
    • To enable the use of standard laboratory incubators for the test.

    Main Methods:

    • The leukocyte migration test medium was formulated with 1% agarose, 0.003 M HEPES, TC medium 199, and 10% horse serum.
    • The pH was adjusted to 7.3 using 2 N NaOH.
    • The optimized medium's performance was evaluated against the intradermal test using purified protein derivative (PPD) as the antigen in 36 subjects.

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    Main Results:

    • The leukocyte migration test performed in the optimized medium showed a strong correlation with the intradermal test.
    • Results obtained using the modified medium were comparable to the reference technique.
    • The addition of HEPES facilitated the performance of the test in a standard incubator.

    Conclusions:

    • The optimized medium provides a reliable and standardized method for the leukocyte migration test.
    • The enhanced leukocyte migration test demonstrates comparable efficacy to the intradermal test for antigen detection.
    • The use of HEPES simplifies the practical application of the leukocyte migration test in clinical laboratories.