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Related Experiment Videos

Serum components stimulate pericardial tissue contraction.

Douglas C Marchion1, Josh J Sticka, Tian Hao Han

  • 1The International Heart Institute of Montana, Missoula 59802, USA.

The Journal of Heart Valve Disease
|May 10, 2002
PubMed
Summary
This summary is machine-generated.

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Serum components greater than or equal to 5 kDa stimulate pericardial tissue contraction and protein synthesis. This process involves heat-stable factors and de-novo protein production, crucial for healing reactions.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Autologous pericardial flap implants in sheep aorta exhibit retraction and fibrosis.
  • An in-vitro model mimics in-vivo pericardial tissue healing reactions.
  • Previous studies identified de-novo protein synthesis as essential for tissue contraction.

Purpose of the Study:

  • To partially characterize serum components stimulating pericardial tissue contraction.
  • To determine the molecular weight range of active serum factors.
  • To assess the stability of these factors to heat and proteases.

Main Methods:

  • Sheep pericardium sections were incubated with fractionated, heat-treated, or protease-treated fetal bovine serum.
  • SDS-PAGE analyzed protein profiles in tissues with and without cycloheximide.

Related Experiment Videos

  • Incubation periods extended up to 14 days.

    • Main Results:

    • Tissue contraction occurred with serum fractions >= 5 kDa.
    • Heat- and protease-treated serum also induced contraction.
    • A specific protein band appeared by day 4, absent when de-novo synthesis was inhibited.

    Conclusions:

    • Serum fractions >= 5 kDa stimulate protein synthesis and pericardial tissue contraction.
    • The active component is heat-stable but partially protease-sensitive.
    • Inhibition of de-novo protein synthesis prevents tissue contraction.