Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stereological length estimation using spherical probes.

P R Mouton1, A M Gokhale, N L Ward

  • 1Laboratory of Neurosciences, Gerontology Research Center (NIA/NIH) Baltimore, MD, U.S.A. School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA 30332-0245, USA. disector@friend.ly.net

Journal of Microscopy
|May 10, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Common genetic variants do not predict recurrent events in coronary heart disease patients.

BMC cardiovascular disorders·2022
Same author

Corrigendum to "Neuron and neuroblast numbers and cytogenesis in the dentate gyrus of aged APP<sub>swe</sub>/PS1<sub>dE9</sub>transgenic mice: Effect of long-term treatment with paroxetine" [Neurobiol Dis. 2017; 104: 50-60].

Neurobiology of disease·2019
Same author

Visualization of atherosclerosis as detected by coronary artery calcium and carotid intima-media thickness reveals significant atherosclerosis in a cross-sectional study of psoriasis patients in a tertiary care center.

Journal of translational medicine·2016
Same author

Influence of a triclosan toothpaste on periodontopathic bacteria and periodontitis progression in cardiovascular patients: a randomized controlled trial.

Journal of periodontal research·2016
Same author

Prevention of antibiotic-associated metabolic syndrome in mice by intestinal alkaline phosphatase.

Diabetes, obesity & metabolism·2016
Same author

Cardiopulmonary exercise testing prior to myeloablative allo-SCT: a feasibility study.

Bone marrow transplantation·2014
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
Same journal

Modular training resources for bioimage analysis.

Journal of microscopy·2026
See all related articles

This study introduces a new stereological method using spherical probes for estimating the total length of linear structures in biological tissues, like nerve fibers, regardless of section orientation.

Area of Science:

  • Neuroscience
  • Stereology
  • Biophysics

Background:

  • Linear structures are vital for physiological functions in biological tissues.
  • Estimating the total length density (Lv) of linear objects is crucial but challenging.
  • Existing stereological methods have limitations regarding section orientation and complexity.

Purpose of the Study:

  • To develop a convenient and straightforward stereological approach for estimating total length (L) and length density (Lv) of linear objects.
  • To overcome the limitations of existing methods requiring specific section orientations or complex counting rules.
  • To validate the new method for estimating the total length of thin nerve fibers in the mouse brain.

Main Methods:

  • Utilized spherical probes for inherent isotropy in sampling.

Related Experiment Videos

  • Employed unbiased fractionator sampling techniques.
  • Applied the method to estimate total length of nerve fibers in the mouse dorsal hippocampus.

    • Main Results:

    • Successfully estimated the total length (L) of linear objects in tissue sections.
    • Demonstrated the method's effectiveness for thin nerve fibers.
    • The new approach simplifies the estimation process for arbitrary section orientations.

    Conclusions:

    • The proposed method offers a convenient and accurate way to estimate total length of linear structures.
    • This technique overcomes the practical challenges associated with traditional stereological approaches.
    • It provides a valuable tool for quantitative analysis of linear elements in biological tissues, particularly in neuroscience research.