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Updated: Aug 14, 2026

Molecular Evolution of the Tre Recombinase
Published on: May 29, 2008
Induced DNA recombination by Cre recombinase protein transduction
Sunil K Joshi1, Kahoko Hashimoto, Pandelakis A Koni
1Institute of Molecular Medicine & Genetics and Department of Medicine, Medical College of Georgia, Augusta 30912, USA.
Abstract:
Cre is a DNA recombinase that recognizes 34 base-pair loxP sites of recombination. We have developed a cell-permeable Cre recombinase, TATCre, that is capable of mediating deletion of loxP-flanked targets by simply adding TATCre to cell cultures. Thus, TATCre allows efficient induced DNA recombination without the use of a Cre recombinase transgene or any other genetic material and should prove useful for the genetic manipulation of a wide variety of cell types that have been engineered to possess loxP sites.
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