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Updated: Aug 19, 2026

Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time
Published on: May 21, 2014
[Evaluation of expression and correlation between P53, BCL-2 and BAX proteins in ovarian neoplasms]
Julia Krystyna Bar1, Ewa Sobańska, Andrzej Popiela
1Katedry i Zakładu Immunologii Klinicznej Akademii Medycznej we Wrocławiu.
Objectives:
Interactions between BCL-2 and other proteins regulating programmed cells death in ovarian carcinomas are poorly understood.
Design:
The evaluation of expression of P53, BCL-2 and BAX proteins in ovarian carcinomas. The associations between oncoproteins studied and the histological structure, grade of differentiation and stage of disease were also analysed.
Material And Methods:
The expression of P53, BCL-2 and BAX was evaluated by immunoperoxidase technique (PAP) in tissue sections and corresponding cyst and/or ascitic fluid cells in individual patients with ovarian carcinoma.
Results:
It was shown that the expression of P53 and BAX was comparable in tissue sections and cyst or ascitic fluid cells of individual patients, however the presence of BCL-2 was detected more frequently in tissue sections. No correlations between markers studied and histological subtypes and grade of carcinoma were found. Taking into account the relationship between P53, BCL-2 and BAX expression, it was possible to classify the studied ovarian carcinomas into four phenotypes. Ovarian cancers with phenotype P53+/BCL-2- were more frequent in III/IV degree FIGO stages whereas the phenotype P53-/BCL-2+ were identified mainly in patients in I/II degree FIGO stages. The progression of disease and death occurred more frequently in groups with phenotype P53+/BCL-2+ and P53-/BCL-2-.
Conclusions:
Our observations indicated that estimation of P53, BCL-2 and BAX protein expression may be important in the choice of the chemotherapy.
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