Related Experiment Video
Updated: Jun 25, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
Published on: September 28, 2012
Role of delayed nuclear envelope breakdown and mitosis in Wolbachia-induced cytoplasmic incompatibility
1Department of Molecular, Cell, and Developmental Biology, 319 Sinsheimer Labs, University of California, Santa Cruz, Santa Cruz, CA 95064, USA.
Abstract:
The bacterium Wolbachia manipulates reproduction in millions of insects worldwide; the most common effect is cytoplasmic incompatibility (CI). We found that CI resulted from delayed nuclear envelope breakdown of the male pronucleus in Nasonia vitripennis. This caused asynchrony between the male and female pronuclei and, ultimately, loss of paternal chromosomes at the first mitosis. When Wolbachia were present in the egg, synchrony was restored, which explains suppression of CI in these crosses. These results suggest that Wolbachia target cell cycle regulatory proteins. A striking consequence of CI is that it alters the normal pattern of reciprocal centrosome inheritance in Nasonia.
Related Concept Videos
Long-patch Base Excision Repair
DNA Damage can Stall the Cell Cycle
Destabilization of Microtubules
Separation of Sister Chromatids
At the onset of anaphase, separase, a proteolytic enzyme, is...
DNA Damage Can Stall the Cell Cycle
Overview Of Cell Separation And Isolation

