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Updated: Oct 1, 2026

Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization
Published on: April 7, 2023
Pronuclear abnormalities and cytoskeletal organization during assisted fertilization in a patient with
V Y Rawe1, S Kopelman, F N Nodar
1Centro de Estudios en Ginecología y Reproducción, CEGyR, Viamonte, Buenos Aires, Argentina. vanerawe@hotmail.com
Purpose:
To analyze the distribution of a tubulins and acetylated alpha tubulins and the chromatin configuration in abnormally fertilized zygotes from a patient with a multifollicular ovarian response after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).
Methods:
Immunofluorescence and phase contrast microscopy was performed in abnormally fertilized zygotes.
Results:
After phase contrast microscopy analysis, immunofluorescence staining was performed in 20 oocytes that developed > or = 3 pronuclei (PN) and karyomeres after IVF-ICSI. Around 80% of the abnormal zygotes from IVF were the consequence of monospermic fertilizations. Retention of the second polar body (PB) and the presumptive split of > or =1 PN within the cytoplasm were the main events present in most oocytes after IVF-ICSI.
Conclusions:
Fluorescence labeling of selected sperm and oocyte components affords a unique view of abnormal fertilized zygotes. Surprisingly, anomalies detected after IVF-ICSI showed similar etiologies in this special group of zygotes.
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