Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Estimation of cyclodextrin affinity to steroids.

S M Khomutov1, I A Sidorov, D V Dovbnya

  • 1G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow region, Russian Federation. khom@ibpm.serpukhov.su

The Journal of Pharmacy and Pharmacology
|May 15, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microminiaturization of Multichannel Multifrequency Radiographs.

Biomedical engineering·2022
Same author

<i>Streptomyces tsukubensis</i> VKM Aс-2618D-an Effective Producer of Tacrolimus.

Applied biochemistry and microbiology·2021
Same author

Features of the immunological profile of blood and urine in patients with post-infectious glomerulonephritis.

Klinicheskaia laboratornaia diagnostika·2021
Same author

Corrigendum to "Comparative analysis of genes encoding key steroid core oxidation enzymes in fast-growing Mycobacterium spp. strains" [J. Steroid Biochem. Mol. Biol. 138 (2013) 41-53].

The Journal of steroid biochemistry and molecular biology·2020
Same author

Microbiological synthesis of stereoisomeric 7(α/β)-hydroxytestololactones and 7(α/β)-hydroxytestolactones.

Applied microbiology and biotechnology·2019
Same author

PCR assays for detection of human astroviruses: In silico evaluation and design, and in vitro application to samples collected from patients in the Netherlands.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology·2018
Same journal

Farnesoid X receptor blockade attenuates morphological damage, intestinal secretion, and prevents mucus loss induced by SARS-CoV-2 spike protein in the mouse intestine.

The Journal of pharmacy and pharmacology·2026
Same journal

Therapeutic strategies of traditional Chinese medicine for chronic heart failure: from active ingredients to herbal formulations.

The Journal of pharmacy and pharmacology·2026
Same journal

Norisoboldine suppresses epithelial-mesenchymal transition in colorectal cancer by simultaneously inhibiting the CXCR4/CXCR7 chemokine receptors and the JAK/STAT3 signaling pathway.

The Journal of pharmacy and pharmacology·2026
Same journal

Development, characterization, and therapeutic evaluation of uniform dezocine-loaded PLGA microspheres for sustained analgesia and cognitive protection.

The Journal of pharmacy and pharmacology·2026
Same journal

Phytochemical constituents, biological activities, toxicological profiles, and current applications of Muntingia calabura L.: an updated review.

The Journal of pharmacy and pharmacology·2026
Same journal

Uterine relaxant effects of Ageratum conyzoides essential oil: influence of hormonal status and calcium modulation.

The Journal of pharmacy and pharmacology·2026
See all related articles

A new nonlinear spectrometric method accurately determines cyclodextrin-steroid complex stability constants (Ks). This technique offers a rapid way to estimate cyclodextrin affinity for hydrophobic molecules.

Area of Science:

  • Analytical Chemistry
  • Supramolecular Chemistry

Background:

  • Cyclodextrins are widely used in drug delivery and separation science.
  • Accurate determination of cyclodextrin-steroid complex stability constants (Ks) is crucial for optimizing their applications.
  • Existing methods for Ks determination can be time-consuming or require specialized equipment.

Purpose of the Study:

  • To develop a novel, rapid, and cost-effective nonlinear spectrometric method for determining cyclodextrin-steroid complex stability constants (Ks).
  • To validate the developed method against the established phase-solubility technique.
  • To investigate the influence of steroid hydrophobicity and cyclodextrin type on Ks values.

Main Methods:

  • A nonlinear spectrometric approach involving simultaneous fitting of two curve types: methyl orange absorbance vs. cyclodextrin concentration, and displacement curves of methyl orange by steroids.

Related Experiment Videos

  • Calculation of stability constants (Ks) using the fitted parameters.
  • Validation using the phase-solubility technique.
  • Determination of Ks for various cyclodextrins (randomly methylated beta-cyclodextrin, 2-hydroxypropyl-beta-cyclodextrin, carboxymethyl-beta-cyclodextrin, sulfobutylether-beta-cyclodextrin) with different steroids.

    • Main Results:

    • The developed nonlinear spectrometric method provides Ks values with a standard deviation less than 10%.
    • The method's accuracy is validated by comparison with phase-solubility data.
    • Ks values are largely unaffected by pH for neutral steroid molecules.
    • Higher Ks values were observed for more hydrophobic steroids.
    • Anionic beta-cyclodextrins demonstrated high affinity for the studied steroids.

    Conclusions:

    • The proposed nonlinear spectrometric method is reliable and accurate for determining cyclodextrin-steroid stability constants.
    • The method is suitable for express estimation of cyclodextrin affinity for hydrophobic substrates due to simple equipment and computational requirements.
    • Findings highlight the impact of steroid hydrophobicity and cyclodextrin structure on complex stability.