Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid fluorescence ratio assay for detecting changes in radiosensitivity.

I Hofland1, B Ramakers, A C Begg

  • 1Division of Experimental Therapy, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.

Radiation Research
|May 15, 2002
PubMed
Summary

A new flow cytometry assay using enhanced green fluorescence protein (EGFP) offers a faster alternative to traditional colony-forming assays for measuring cancer therapy drug and radiation sensitivity in preclinical studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A joint physics and radiobiology DREAM team vision - Towards better response prediction models to advance radiotherapy.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2024
Same author

Cellular Radiosensitivity of Soft Tissue Sarcoma.

Radiation research·2021
Same author

Study protocols of three parallel phase 1 trials combining radical radiotherapy with the PARP inhibitor olaparib.

BMC cancer·2019
Same author

Micro cone beam computed tomography for sensitive assessment of radiation-induced late lung toxicity in preclinical models.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology·2019
Same author

Calcification of the left auricle; report of a case.

The New Zealand medical journal·2010
Same author

OBSERVATIONS ON THE VACCINE TREATMENT OF A SERIES OF CASES OF STAPHYLOCOCCIC INFECTIONS.

British medical journal·2010

Area of Science:

  • Oncology
  • Cell Biology
  • Biotechnology

Background:

  • The colony-forming assay is the gold standard for assessing radiosensitivity in cancer research.
  • This traditional assay is time-consuming, laborious, and not ideal for high-throughput screening.
  • There is a need for more efficient methods to evaluate drug and radiation sensitivity in preclinical cancer studies.

Purpose of the Study:

  • To develop and validate a novel, rapid assay for measuring modifications in radiosensitivity.
  • To provide a more efficient alternative to the colony-forming assay for preclinical cancer therapy studies.

Main Methods:

  • A new assay utilizing enhanced green fluorescence protein (EGFP) and flow cytometry was developed.
  • Cells were co-cultured as mixed populations of a standard nonfluorescent cell line and a genetically modified EGFP-expressing cell line.

Related Experiment Videos

  • The ratio of fluorescent to nonfluorescent cells was measured before and after irradiation to determine changes in radiosensitivity.

    • Main Results:

    • The assay successfully detected changes in radiosensitivity when cells expressed dominant-negative peptides that inhibit DNA repair.
    • The EGFP-based assay demonstrated its ability to validate modifications in radiosensitivity in A549 human lung cells.

    Conclusions:

    • The EGFP-based flow cytometry assay is a viable, efficient alternative to the colony-forming assay for assessing radiosensitivity.
    • This assay is particularly suitable for genetic manipulation studies and can be adapted for various reporters.