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Related Experiment Videos

Polyploid measles virus with hexameric genome length.

Monika Rager1, Sompong Vongpunsawad, William Paul Duprex

  • 1Molecular Medicine Program, Mayo Clinic, Guggenheim 1838, 200 First Street SW, Rochester, MN 55905, USA.

The EMBO Journal
|May 15, 2002
PubMed
Summary

Measles virus mutants can package multiple genomes, with complementary RNA genomes ensuring efficient replication. Polyploid virus particles are produced without impacting infectivity or growth.

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Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Most viruses package a single genome, but parainfluenza viruses may package multiple genomes.
  • Measles virus (a paramyxovirus) typically has a single-stranded RNA genome.

Purpose of the Study:

  • To characterize a measles virus mutant that packages multiple genomes.
  • To investigate the functional implications of polyploid viral particles.

Main Methods:

  • Characterization of a stable measles virus mutant with multiple genomes.
  • Analysis of RNA sequences, translation, and polymerase activity.
  • Assessment of infection kinetics and particle infectivity.

Main Results:

  • A measles virus mutant was identified that packages at least two complementary RNA genomes.

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  • One genome contained mutations restoring attachment protein function and ensuring accurate RNA encapsidation.
  • Polyploid particles were produced in standard infections without compromising viral growth or infectivity.

    • Conclusions:

    • Measles virus particles can efficiently accommodate multiple RNA genomes of varying sizes.
    • This packaging mechanism suggests a pathway for the evolution of segmented viral genomes.