Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rapid, quantitative immunofunctional assay for measuring human leptin.

Jürgen Kratzsch1, Antje Berthold, Alexander Lammert

  • 1Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital, University of Leipzig, Germany. Kraj@medizin.uni-leipzig.de

Hormone Research
|May 15, 2002
PubMed
Summary

A new immunofunctional assay (IFA) accurately measures leptin levels by utilizing the soluble leptin receptor (sOB-R). This rapid, sensitive assay shows high correlation with BMI and existing RIA methods, offering a valuable tool for leptin research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Lipid Droplet-Associated Hydrolase Is Associated With Obesity and Adipose Tissue Inflammation in Children.

Obesity (Silver Spring, Md.)·2026
Same author

Age- and sex-specific reference intervals for 11-oxygenated androgens from infancy throughout childhood and adulthood.

Clinical chemistry and laboratory medicine·2026
Same author

Mild Variations in Thyroid Hormone Serum Concentrations Show Associations with the ET 6-6-R Developmental Test: Results from a Population-Based Pediatric Cohort in Germany.

Hormone research in paediatrics·2025
Same author

New sex hormone-binding globulin and estimated free testosterone reference intervals for children and adolescents: The effects of age, sex, BMI and oral contraceptives.

Clinica chimica acta; international journal of clinical chemistry·2025
Same author

Reference Values for Serum Leptin Levels in Children, Adolescents, and Adults With Normal Weight, Overweight, and Obesity.

The Journal of clinical endocrinology and metabolism·2025
Same author

Effect of sex, pubertal stage, body mass index, oral contraceptive use, and C-reactive protein on vitamin D binding protein reference values.

Frontiers in endocrinology·2025

Area of Science:

  • Biochemistry
  • Endocrinology
  • Assay Development

Background:

  • Leptin plays a crucial role in metabolic regulation.
  • Accurate leptin measurement is essential for understanding physiological and pathological states.
  • Existing assays may not fully differentiate between functionally active and inactive leptin forms.

Purpose of the Study:

  • To develop and validate a rapid, sensitive, and quantitative in vitro assay for leptin.
  • To leverage the leptin receptor's binding capability for assay development.
  • To assess the assay's ability to differentiate physiologically active leptin.

Main Methods:

  • Developed an immunofunctional assay (IFA) using immobilized soluble leptin receptor (sOB-R).
  • Quantified leptin in serum samples via sequential antibody binding and enzyme-linked detection.

Related Experiment Videos

  • Clinically validated the IFA by comparing results with a radioimmunoassay (RIA) in adolescents and adults.

    • Main Results:

    • The IFA demonstrated functional sensitivity of 0.4 ng/ml, comparable to RIA.
    • High correlation between IFA and RIA measurements (r = 0.97) and with Body Mass Index (BMI) (r = 0.72).
    • Intra- and interassay coefficients of variation were below 12.5% for both methods.

    Conclusions:

    • The newly developed IFA shows minimal differences compared to conventional RIA.
    • Further studies are needed to explore IFA's utility in states of leptin resistance.
    • Investigating the biological relevance of potential discrepancies in leptin resistance states is warranted.