Related Experiment Video
Updated: Oct 1, 2026

Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining
Published on: April 21, 2023
Nongenomic effects of corticosteroids on ion transport by stria vascularis
1Kansas State University, Department of Anatomy and Physiology, Manhattan, Kans 66506-5802, USA.
Abstract:
Stria vascularis electrogenically secretes potassium into endolymph and this secretion is known to be under acute control of several hormone receptors including purinergic, adrenergic and muscarinic receptors. Recently, chronic application of glucocorticosteroid hormones in autoimmune mice was reported to restore both hearing and normal strial morphology. The purpose of this study was to investigate the acute nongenomic effects of glucocorticoids and mineralocorticoid on the short circuit current (I(sc,probe)) across isolated stria vascularis of gerbils using the voltage-sensitive vibrating probe. Application of prednisolone (0.1-30 microM) caused an increase of I(sc,probe) in a dose-dependent manner with an EC50 of 1.1 +/- 0.2 microM (n = 5). Hydrocortisone (10 microM, n = 5) and dexamethasone (10 microM, n = 5) also increased I(sc,probe), but the response was transient and the response rate was faster than for prednisolone. By contrast, aldosterone (10 microM, n = 6) caused a transient decrease of I(sc,probe )within seconds. These results suggest that prednisolone increased secretion of K+ via a nongenomic mechanism in the range of therapeutic plasma concentrations and that this stimulatory effect of glucocorticoids is specific since the mineralocorticoid aldosterone caused a distinctly different response.
More Related Videos
08:46Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
Published on: September 1, 2015
09:36Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model
Published on: August 29, 2012
Related Concept Videos
Pore Transport and Ion-Pair Transport
Pore transport, also known as convective transport, is a process where small molecules like urea, water, and sugars rapidly cross cell membranes as though there were channels or pores in the membrane. Although direct microscopic evidence is limited but the concept of pores or channels is widely accepted based on physiological evidence. Despite the lack of direct microscopic...
Hormones of the Adrenal Glands
The adrenal cortex, a powerhouse of hormone synthesis, generates over two dozen corticosteroid hormones. The zona glomerulosa produces mineralocorticoids, exemplified by aldosterone, influencing the electrolyte composition of body fluids. The synthesis of glucocorticoids such as cortisol and corticosterone...
Antihypertensive Drugs: Action of β1 Blockers
Antihypertensive Drugs: Potassium-Sparing Diuretics
Antihypertensive Drugs: Action of Diuretics
Antiarrhythmic Drugs: Class I Agents as Sodium Channel Blockers
Class 1A Antiarrhythmic Drugs: These drugs work by moderately blocking sodium channels,...