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Detergent extract from chlamydial elementary bodies used as antigen for enzyme-linked immunosorbent assay
M A Quevedo Diaz1, J Melnicáková, J Kazár
1Institute of Virology, Slovak Academy of Sciences, Dúbravská 9, 84245 Bratislava, Slovak Republic.
Diagnostic Microbiology and Infectious Disease
|May 15, 2002
Abstract:
Soluble antigen (SA) from chlamydial elementary bodies (EBs) was extracted with N-lauroylsarcosine. The extracted SA composed of lipopolysaccharide (LPS) and proteins was compared with EBs using an enzyme-linked immunosorbent assay (ELISA). Patient sera from natural chlamydial infections exhibited ELISA mean absorbance (A(492) and A(405/650)) values 2-5 times higher with SA than with EBs, resulting in a better discrimination between positive and negative human sera.