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Analysis of heat-shock related gene expression in head-and-neck cancer using cDNA arrays
Norihiko Narita1, Ichiro Noda, Toshio Ohtsubo
1Department of Otorhinolaryngology, Fukui Medical University, Matsuoka, Fukui 910-1193, Japan. norihiko@fmsrsa.fukui-med.ac.jp
Purpose:
The aim of the present study was to identify genes regulated in the early response to heat-shock in human head-and-neck cancer cells using a cDNA array.
Methods And Materials:
IMC-3 cells were heat-shocked at 44 degrees C for 30 min, then incubated for 6 h. After 6 h incubation, mRNAs were extracted. Early gene expressions in IMC-3 cells were analyzed using a cDNA array after heat-shock. In addition, reverse transcription-polymerase chain reaction (RT-PCR) was performed to identify the expressions of mRNA for confirming features by cDNA array analysis in several other cell lines (KB, T3M-1, SCC-TF) as well as in IMC-3 cells.
Results:
Twenty-eight genes were found to be induced or upregulated by heat-shock in IMC-3 cells. These included genes involved in the apoptosis (e.g., CC3, caspase10), tumor invasion (e.g., CC3, TIMP-3), cell cycle checkpoint control (e.g., DP-1, CDC25A), signal transduction (e.g., MEK1) as well as genes associated with heat stress (e.g., Hsp70B', Hsp40). Gene expressions of CC3 and MEK1 were recognized to be induced by heat-shock in pharyngeal cancer cells (KB, T3M-1) and lingual cancer cells (SCC-TF).
Conclusion:
The observed alteration in gene expression may play a role in various biochemical pathways of cancer cells exposed to heat-shock.