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Updated: Oct 1, 2026

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
Published on: January 20, 2019
In vitro and in vivo plasticity of Pax5-deficient pre-B I cells
Antonius G Rolink1, Christoph Schaniel, Ludovica Bruno
1Basel Institute for Immunology, Basel, Switzerland. antonius.rolink@unibas.ch
Abstract:
In mice deficient for the transcription factor Pax-5, B cell development is blocked at the pre-B I cell stage. Like wild type, Pax-5-/- pre-B I cells can be grown long-term in vitro in the presence of stromal cells and IL-7. However, unlike their wild type in vitro grown counterparts, Pax-5-/- pre-B I cells posses an extraordinary developmental plasticity showing hematopoeitic stem cell features such as multipotency and self renewing capacity. Here we review and discuss this in vitro and in vivo plasticity of Pax-5-/- pre-B I cells.
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