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T type 1/type 2 subsets balance in B-cell chronic lymphocytic leukemia--the three-color flow cytometry analysis

Monika Podhorecka1, Anna Dmoszynska, Jacek Rolinski

  • 1Department of Clinical Immunology, University School of Medicine in Lublin, Jaczewskiego 8, 20-950, Lublin, Poland. mpodhor@asklepios.am.lukin.pl

Leukemia Research
|May 15, 2002
PubMed

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...