Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

c-myc is required for osteoclast differentiation.

R Battaglino1, D Kim, J Fu

  • 1Department of Cytokine Biology, The Forsyth Institute, and Harvard School of Dental Medicine, Boston, Massachusetts 02115, USA.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|May 15, 2002
PubMed
Summary

The proto-oncogene c-myc is essential for osteoclast formation, a process crucial for bone remodeling. This study demonstrates that c-myc is a key downstream target of RANKL signaling in osteoclastogenesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimizing typhoid fever case definitions by combining serological tests in a large population study in Hechi City, China.

Epidemiology and infection·2007
Same author

Intra-arterial thrombolysis for acute stroke in patients 80 and older: a comparison of results in patients younger than 80 years.

AJNR. American journal of neuroradiology·2007
Same author

Influence of hepatitis C on renal function after liver transplantation.

Transplantation proceedings·2006
Same author

A comparison of twice-daily exenatide and biphasic insulin aspart in patients with type 2 diabetes who were suboptimally controlled with sulfonylurea and metformin: a non-inferiority study.

Diabetologia·2006
Same author

Hypermethylation of CpG island loci and hypomethylation of LINE-1 and Alu repeats in prostate adenocarcinoma and their relationship to clinicopathological features.

The Journal of pathology·2006
Same author

Endovascular mechanical clot retrieval in a broad ischemic stroke cohort.

AJNR. American journal of neuroradiology·2006

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Receptor activator of nuclear factor kappaB (RANKL) is a key cytokine in osteoclast formation.
  • Downstream signaling pathways of RANKL in osteoclastogenesis are not fully understood.

Purpose of the Study:

  • To identify genes regulated by RANKL during osteoclast differentiation.
  • To elucidate the role of c-myc in RANKL-induced osteoclastogenesis.

Main Methods:

  • RAW 264.7 mouse monocytes were used as a model for osteoclast differentiation.
  • RANKL induction was used to generate osteoclast-like cells (OCLs).
  • Gene expression analysis (Northern Blot) and dominant-negative c-myc expression were employed.

Main Results:

Related Experiment Videos

  • RANKL induced the formation of OCLs expressing osteoclast-specific markers and resorption activity.
  • The proto-oncogene c-myc was significantly upregulated in RANKL-induced OCLs.
  • Inhibition of c-myc expression blocked RANKL-induced OCL formation and reduced mRNA levels of TRAP and cathepsin K.

Conclusions:

  • c-myc is a critical downstream target of RANKL signaling.
  • c-myc expression is required for RANKL-induced osteoclastogenesis and the differentiation of osteoclasts.