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Purification and properties of a lectin from ascomycete mushroom, Ciborinia camelliae
Yumi Otta1, Koh Amano, Kano Nishiyama
1Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University, 271-8510, Matsudo, Japan.
Phytochemistry
|May 16, 2002
Abstract:
A lectin was isolated from an ascomycete mushroom, Ciborinia camelliae which was specific to N-acetyl-D-galactosamine. On SDS-polyacrylamide gel electrophoresis; this lectin gave a single band of approximately 17-kDa in the presence of 2-mercaptoethanol, but formed dimers, trimers and tetramers in its absence. Amino acid analysis revealed the lectin contained two cysteines and no methionine. The N-terminal sequence was determined up to residue 21, and no homologous proteins including other ascomycete lectins were found.