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Updated: Aug 18, 2026
Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae
Published on: February 16, 2015
Plasma membrane injury induced by nonyl gallate in Saccharomyces cerevisiae
1Department of Environmental Science, Policy and Management, University of California, Berkeley, California, USA.
Aims:
The aim was to investigate the antifungal actions of nonyl gallate against Saccharomyces cerevisiae ATCC 7754.
Methods And Results:
The maximum potency of both the growth inhibitory and the fungicidal effect against the yeast strain was found in nonyl gallate among n-alkyl gallates tested. Nonyl gallate induced ROS generation dose-dependently in growing cells. This ester rapidly killed yeast cells even when cell division was restricted by cycloheximide. This ester inhibited glucose-induced medium acidification and promoted the efflux of intracellular potassium ions in a nongrowing condition. Moreover, nonyl gallate induced a leakage of calcein from artificially prepared liposomes to a greater extent than dodecyl gallate did.
Conclusions:
These results suggested nonyl gallate injured plasma membrane of S. cerevisiae, resulting in its exhibition of fungicidal effect accompanying with a leakage of intracellular materials from the cells.
Significance And Impact Of The Study:
Our study reveals new knowledge on the antifungal actions of nonyl gallate against S. cerevisiae. When nonyl gallate is applied as a food preservative, the level of its addition to foods may be reduced because of its potent antifungal activity compared with weak acids including sorbic acid and benzoic acid.
