Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glucocorticoids: do we know how they work?

Jeremy Saklatvala1

  • 1Kennedy Institute of Rheumatology Division, Faculty of Medicine, Imperial College of Science, Technology and Medicine, London, UK. j.saklatvala@ic.ac.uk

Arthritis Research
|May 16, 2002
PubMed
Summary

Glucocorticoid hormones reduce inflammation through molecular mechanisms involving gene expression changes. Understanding these pathways could lead to targeted anti-inflammatory drugs with fewer side effects.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Effects of Age and Cell Isolation on Collagen II Synthesis by Articular Chondrocytes: Evidence for Transcriptional and Posttranscriptional Regulation.

BioMed research international·2020
Same author

Connective tissue growth factor contributes to joint homeostasis and osteoarthritis severity by controlling the matrix sequestration and activation of latent TGFβ.

Annals of the rheumatic diseases·2018
Same author

Rapid Activation of Transforming Growth Factor β-Activated Kinase 1 in Chondrocytes by Phosphorylation and K<sup>63</sup> -Linked Polyubiquitination Upon Injury to Animal Articular Cartilage.

Arthritis & rheumatology (Hoboken, N.J.)·2016
Same author

Acute Molecular Changes in Synovial Fluid Following Human Knee Injury: Association With Early Clinical Outcomes.

Arthritis & rheumatology (Hoboken, N.J.)·2016
Same author

Brief Report: JNK-2 Controls Aggrecan Degradation in Murine Articular Cartilage and the Development of Experimental Osteoarthritis.

Arthritis & rheumatology (Hoboken, N.J.)·2015
Same author

Dual-Specificity Phosphatase 1 and Tristetraprolin Cooperate To Regulate Macrophage Responses to Lipopolysaccharide.

Journal of immunology (Baltimore, Md. : 1950)·2015

Area of Science:

  • Molecular Biology
  • Pharmacology
  • Immunology

Background:

  • Glucocorticoid hormones mediate anti-inflammatory effects through complex molecular pathways.
  • The precise mechanisms underlying both therapeutic and adverse effects of glucocorticoids are not fully elucidated.
  • Glucocorticoid receptors interact with DNA and other transcription factors to modulate gene expression.

Purpose of the Study:

  • To investigate the molecular mechanisms by which glucocorticoid hormones exert anti-inflammatory actions.
  • To differentiate the pathways responsible for therapeutic versus side effects of glucocorticoids.
  • To explore the potential for developing selective glucocorticoid receptor modulators.

Main Methods:

  • Analysis of glucocorticoid receptor binding to DNA.
  • Investigation of glucocorticoid receptor interference with transcription factors like activator protein-1 and NF-kappaB.
  • Examination of glucocorticoid-induced gene expression, including the protein inhibitor of NF-kappaB.
  • Assessment of glucocorticoid inhibition of mitogen-activated protein kinase signaling pathways.

Main Results:

  • Glucocorticoids modulate gene expression via direct DNA binding or interference with transcription factors.
  • The liganded glucocorticoid receptor inhibits key inflammatory mediators, including activator protein-1 and NF-kappaB.
  • Glucocorticoids induce anti-inflammatory genes and inhibit inflammatory signaling pathways like MAPK, dependent on new gene expression.

Conclusions:

  • Glucocorticoid anti-inflammatory effects are mediated by distinct molecular mechanisms involving transcription factor modulation and signaling pathway inhibition.
  • Identifying the specific pathways for anti-inflammatory actions versus side effects is crucial.
  • This knowledge may enable the development of novel steroid-related drugs for targeted anti-inflammatory therapy.

Related Experiment Videos