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Related Experiment Videos

Neutrophil activation by heme: implications for inflammatory processes.

Aurélio V Graça-Souza1, Maria Augusta B Arruda, Marta S de Freitas

  • 1Departamento de Farmacologia, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Brazil. avsouza@bioqmed.ufrj.br

Blood
|May 16, 2002
PubMed
Summary

Free heme (ferriprotoporphyrin IX) acts as a proinflammatory molecule, activating neutrophils. This finding is relevant for conditions involving cell damage and inflammation, such as hemolytic anemia.

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Area of Science:

  • Biochemistry
  • Immunology
  • Cell Biology

Background:

  • Heme is an iron-containing compound found in cells.
  • Heme release increases during diseases like malaria, hemolytic anemia, trauma, and hemorrhage.
  • Free heme can be dangerous and act as a proinflammatory molecule.

Purpose of the Study:

  • To investigate the role of free ferriprotoporphyrin IX (hemin) as a proinflammatory molecule.
  • To determine hemin's ability to activate neutrophil responses in vivo and in vitro.

Main Methods:

  • Hemin injection into rat pleural cavity to assess neutrophil migration.
  • In vitro studies on human neutrophils: chemotaxis, actin polymerization, interleukin-8 expression (RT-PCR), oxidative burst, and protein kinase C activation.
  • Dose-dependent and concentration-dependent analyses were performed.

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Main Results:

  • Hemin induced dose-dependent neutrophil migration in vivo.
  • Hemin promoted human neutrophil chemotaxis, actin polymerization, and interleukin-8 expression in vitro.
  • Hemin triggered the oxidative burst and activated protein kinase C in human neutrophils.

Conclusions:

  • Hemin acts as a proinflammatory agent.
  • Hemin can induce polymorphonuclear neutrophil activation.
  • These findings are relevant to clinical conditions involving hemolysis or hemoglobinemia.