Related Experiment Videos
DNA microarray assessment of putative Borrelia burgdorferi lipoprotein genes
Fang Ting Liang1, F Kenneth Nelson, Erol Fikrig
1Section of Rheumatology, Department of Internal Medicine, School of Medicine, Yale University, New Haven, Connecticut 06520-8031, USA.
Infection and Immunity
|May 16, 2002
Abstract:
A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstrating the degree to which the individual putative lipoprotein genes were conserved among the genospecies. These data show that a DNA microarray can globally examine the genes encoding B. burgdorferi lipoproteins.