Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

BRCA1 regulates the interferon gamma-mediated apoptotic response.

Heather N Andrews1, Paul B Mullan, Stewart McWilliams

  • 1Department of Oncology, Cancer Research Centre, The Queen's University Belfast, Belfast BT9 7AB, Northern Ireland.

The Journal of Biological Chemistry
|May 16, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic monitoring of antibody drug conjugates targeting TROP2 or HER2 in breast cancer using circulating tumor cells.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Safety and antitumour activity of ipatasertib combined with endocrine therapy and a CDK4/6 inhibitor in HR+/HER2- metastatic breast cancer (TAKTIC): a single-centre, open-label, phase 1b trial.

The Lancet. Oncology·2026
Same author

Author Correction: HER2 expression identifies dynamic functional states within circulating breast cancer cells.

Nature·2026
Same author

ATR Safeguards Epithelial-to-Mesenchymal Transition by Countering R-loops and Enabling Transcription Reprogramming.

The Journal of clinical investigation·2026
Same author

Circulating Tumor Cells Predict Response to the DLL3-Targeting Bispecific Antibody Tarlatamab.

Cancer discovery·2026
Same author

TMPO promotes cellular dissemination and metastasis in circulating tumor cells.

bioRxiv : the preprint server for biology·2026

This study reveals that BRCA1 (Breast Cancer gene 1) regulates interferon gamma signaling. BRCA1 influences interferon gamma-induced apoptosis, highlighting its role in immune response pathways.

Area of Science:

  • Molecular Biology
  • Cancer Genetics
  • Immunology

Background:

  • BRCA1 is a critical tumor suppressor gene involved in transcriptional regulation.
  • Understanding BRCA1's downstream targets is key to elucidating its function in cancer suppression.
  • Interferon signaling pathways play a significant role in cellular defense and tumor suppression.

Purpose of the Study:

  • To identify downstream targets of BRCA1 using inducible expression systems.
  • To investigate the interplay between BRCA1 and interferon signaling pathways.
  • To explore BRCA1's role in interferon gamma-mediated apoptosis.

Main Methods:

  • Generation of cell lines with inducible BRCA1 expression.
  • Oligonucleotide array-based gene expression profiling to identify differentially expressed genes.

Related Experiment Videos

  • Northern blot analysis to validate target gene regulation.
  • Functional studies in BRCA1-mutant cell lines (HCC1937) with and without BRCA1 reconstitution.

    • Main Results:

    • Inducible BRCA1 expression up-regulated 11 interferon-regulated genes.
    • BRCA1 synergistically enhanced interferon gamma-induced expression of IRF-7, MxA, and ISG-54.
    • Interferon gamma-mediated induction of IRF-7 and MxA was impaired in BRCA1-mutant cells and restored upon BRCA1 reintroduction.
    • Reconstitution of BRCA1 sensitized cells to interferon gamma-induced apoptosis.

    Conclusions:

    • BRCA1 is identified as a component of the interferon gamma signaling pathway.
    • BRCA1 plays a regulatory role in interferon gamma-mediated apoptosis.
    • These findings suggest a novel mechanism by which BRCA1 contributes to tumor suppression and immune regulation.