[Direct fluorescence microscopy in the diagnosis of glomerulonephritis]

The use of the method of direct immunofluorescence (DIF) in the examination of the renal biopsy tissue, differentiation of various forms of glomerulonephritis, identification of immunopathogenetic mechanisms of the disease and mediators of immune reaction, are presented. In the study are described the characteristics findings of DIF in differentiation of morphologic groups of glomerulonephritis, made after light microscopy (LM). Here are presented the type and localisation of immune deposits within the kidney tissue (glomerules, blood vessels, tubules and interstitium). It was performed 52 renal biopsies in the period between 1997 and 2001 year, at the Institute of Nephrology in Sarajevo, than divided after DIF and LM in 11 different groups, with dominant membranous glomerulopathy (11 cases), diffuse mesangioproliferative with IgA nephropathy (9 cases) and minimal change glomerulonephritis (9 cases), between them. It was described the method of renal biopsy, the preservation and preparation of biopsy spacemen, and the act of fluorescence microscopy.