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Molecular alterations in chronic ulcerative colitis-associated and sporadic hyperplastic polyps: a comparative
Robert D Odze1, Tom Brien, Charlotte A Brown
1Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
The American Journal of Gastroenterology
|May 17, 2002
Summary
Colonic hyperplastic polyps in chronic ulcerative colitis (CUC) are genetically similar to sporadic hyperplastic polyps. This finding supports the theory that these polyps may have neoplastic potential.
Area of Science:
- Gastroenterology
- Molecular Biology
- Oncology
Background:
- Colonic hyperplastic polyps are of growing interest due to K-ras mutations.
- Hyperplastic polyps can occur in chronic ulcerative colitis (CUC), but their biological similarity to sporadic polyps is unknown.
Purpose of the Study:
- To compare the molecular profiles of CUC-associated hyperplastic polyps with sporadic hyperplastic polyps.
- To investigate the neoplastic potential of colonic hyperplastic polyps.
Main Methods:
- Polymerase chain reaction (PCR) was used to analyze genetic alterations (APC, 3p, p53, p16, K-ras mutations) in hyperplastic polyps.
- Immunohistochemistry evaluated proliferation markers (Ki67/MIB-1) and p27 expression.
Main Results:
- CUC-associated hyperplastic polyps (47% genetic alterations) showed similar genetic profiles to sporadic hyperplastic polyps (33%).
- Both types of polyps exhibited similar MIB-1 proliferation indices and a high rate of p27 expression loss (46-64%).
- Mutations found in CUC hyperplastic polyps were not present in adjacent inflamed mucosa.
Conclusions:
- CUC-associated hyperplastic polyps are genotypically similar to sporadic hyperplastic polyps.
- The molecular alterations found in hyperplastic polyps lend support to their potential for neoplastic transformation.