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[Gene diagnosis of hemophilia B by multiple STR analysis]
Xiangfan Liu1, Xuefeng Wang, Qishi Fan
1Shanghai Ruijin Hospital, Shanghai Institute of Hematology, Shanghai 200025, China.
Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi
|May 17, 2002
Abstract
Objective:
To establish a simple, rapid genetic diagnostic system for haemophilia B.
Methods:
The polymorphisms of eight STR loci in 87 normal persons and 8 haemophilia B families were assayed by PCR and genescan, and the linkage relations were analysed.
Results:
Six of the eight STR loci can provide genetic information for haemophilia B, and the heterozygosity is 0.50 approximately 0.83, PIC 0.39 approximately 0.80, and DP 0.66 approximately 0.94.
Conclusion:
Combination of multiple STR loci analysis could be effective method for genetic diagnosis of haemophilia B.