Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nitric oxide synthase expression after human brain contusion.

Caroline Gahm1, Staffan Holmin, Tiit Mathiesen

  • 1Department of Clinical Neuroscience, Section of Neurosurgery, Karolinska Institute, Stockholm, Sweden.

Neurosurgery
|May 23, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CGRP-targeted migraine treatment and early pathophysiology in experimental subarachnoid hemorrhage.

The journal of headache and pain·2026
Same author

Ethical aspects of waiting lists in neurosurgery.

Acta neurochirurgica·2026
Same author

The Ki-67 proliferation index and recurrence risk of intracranial meningioma: a multicenter, retrospective cohort study of 5,050 patients.

Acta neurochirurgica·2026
Same author

A Supportive Group Intervention for Caregivers to Patients Diagnosed With Glioblastoma: Protocol for the SUGRI Study.

JMIR research protocols·2026
Same author

Distribution of Sentinel Nodes in Non-Parotid Salivary Gland Tumors-A Feasibility Study.

Cancer reports (Hoboken, N.J.)·2026
Same author

Ethical issues in neurosurgery - A scoping review from the EANS Ethico-legal committee.

Brain & spine·2026

Inducible nitric oxide synthase (iNOS) expression increases in human brain tissue after contusional trauma. This occurs in various brain cells over time, supporting the relevance of animal models for studying traumatic brain injury.

Area of Science:

  • Neuroscience
  • Pathophysiology
  • Traumatic Brain Injury

Background:

  • Nitric oxide (NO) is a key mediator in brain function and traumatic brain injury (TBI).
  • Previous studies suggest altered nitric oxide synthase (NOS) isoform expression post-TBI.

Purpose of the Study:

  • To investigate the cellular sources and tissue distribution of nitric oxide production in human patients with contusional brain injuries.

Main Methods:

  • Immunohistochemical analysis of contused brain tissue from eight human patients.
  • Double-staining assays to identify cellular sources of different NOS isoforms.

Main Results:

  • Inducible NOS (iNOS) expression increased within 6 hours post-trauma, peaking at 8-23 hours.

Related Experiment Videos

  • iNOS was found in neurons, macrophages, neutrophils, astrocytes, and oligodendrocytes, with varying cellular sources over time.
  • No significant changes in neuronal or endothelial NOS isoforms were observed compared to controls.

    • Conclusions:

    • iNOS expression is upregulated in a time-dependent manner following human contusional brain trauma.
    • These human findings align with experimental data in animal models, validating their relevance for TBI research and therapeutic development.