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Updated: Jul 6, 2026
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Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
Published on: December 14, 2017
Protein mapping in rat basophilic leukaemia cells.
K M Carroll1, E M Carey, B A Helm
1Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2UH, UK. k.carroll@sheffield.ac.uk
Summary
Researchers explored the rat basophilic leukaemia (RBL) cell proteome to understand mast cell function in allergic responses. Advanced staining and immunoblotting techniques were used to identify proteins and aid database expansion.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Mast cells play a crucial role in allergic responses.
- Rat basophilic leukaemia (RBL) cells serve as a model for studying mast cell function.
- Understanding the mast cell proteome is essential for elucidating allergic mechanisms.
Purpose of the Study:
- To further decipher the mast cell proteome using advanced techniques.
- To identify and characterize proteins within RBL cells.
- To contribute to existing phosphoprotein and two-dimensional gel databases.
Main Methods:
- Utilized rat basophilic leukaemia (RBL) cells as a model system.
- Employed various staining and immunoblotting procedures.
- Demonstrated technique sensitivity using an anti-phosphotyrosine antibody.
Main Results:
- Identified several protein spots with specific pI/M(r) coordinates.
- Showcased the effectiveness of staining and immunoblotting for proteome analysis.
- Validated the sensitivity of the applied methods.
Conclusions:
- Advanced proteomic techniques can effectively analyze the mast cell proteome.
- The findings contribute valuable data to protein and phosphoprotein databases.
- Further characterization of the mast cell proteome enhances understanding of allergic responses.
