Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Direct sensitivity test of the MB/BacT system.

Angela Maria Werneck Barreto1, Joselba Borges Melo Araujo, Reginalda Ferreira de Melo Medeiros

  • 1Laboratório de Tuberculose, Centro de Referência Prof. Hélio Fraga, Rio de Janeiro, RJ, 22710-550, Brasil. werneck@openlink.com.br

Memorias Do Instituto Oswaldo Cruz
|May 23, 2002
PubMed
Summary

The Organon Teknika MB/BacT system accurately detects tuberculosis drug sensitivity using direct drug susceptibility testing. Rifampin, isoniazid, and pyrazinamide showed high sensitivity and specificity in this study.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic Characterization and Population Structure of Drug-Resistant <i>Mycobacterium tuberculosis</i> Isolated from Brazilian Patients Using Whole-Genome Sequencing.

Antibiotics (Basel, Switzerland)·2024
Same author

Phenotypic and Genotypic Drug Susceptibility Assessment of <i>Mycobacterium bovis</i> Bacillus Calmette-Guérin Clinical Strains.

Infection and drug resistance·2021
Same author

Characterization of Mycobacterium tuberculosis var. africanum isolated from a patient with pulmonary tuberculosis in Brazil.

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases·2020
Same author

Discovery of a novel Mycobacterium asiaticum PRA-hsp65 pattern.

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases·2019
Same author

Drug susceptibility profile of Mycobacterium kansasii clinical isolates from Brazil.

Journal of global antimicrobial resistance·2019
Same author

Integrated analysis of ethionamide resistance loci in Mycobacterium tuberculosis clinical isolates.

Tuberculosis (Edinburgh, Scotland)·2018

Area of Science:

  • Clinical microbiology
  • Infectious diseases
  • Antimicrobial resistance

Background:

  • Tuberculosis (TB) treatment requires accurate drug susceptibility testing (antibiogram).
  • Direct drug susceptibility testing methods aim to provide faster results than traditional indirect methods.
  • The Organon Teknika MB/BacT system is a nonradiometric automated system for microbial detection.

Purpose of the Study:

  • To evaluate the direct-method drug sensitivity testing performance of the Organon Teknika MB/BacT system for principal tuberculosis treatment drugs.
  • To compare the MB/BacT system's results against the BACTEC 460 TB system as the gold standard.

Main Methods:

  • 50 sputum samples from pulmonary tuberculosis patients were tested.
  • Samples underwent decontamination and were inoculated into MB/BacT bottles containing specific anti-TB drugs (Rifampin, Isoniazid, Pyrazinamide, Ethambutol, Ethionamide, Streptomycin).

Related Experiment Videos

  • Results were compared with the indirect method using the BACTEC 460 TB system.

    • Main Results:

    • The Rifampin test demonstrated 100% sensitivity and 100% specificity.
    • Isoniazid and Pyrazinamide also showed high sensitivity and specificity.
    • 92% of samples yielded results within 30 days, aligning with CDC recommendations.
    • No contamination was observed in the inoculated flasks.

    Conclusions:

    • The Organon Teknika MB/BacT system is a reliable and rapid automated nonradiometric method for tuberculosis drug susceptibility testing.
    • The direct-method drug sensitivity testing in the MB/BacT system provides accurate results for key anti-TB drugs.