[Determination of steroidal saponins in Rhizoma paridis by RP-HPLC]

A reversed phase HPLC method for the separation and determination of nine steroidal saponins in Rhizoma paridis is described. The column was Symmetry C8, mobile phase was acetonitrile-water (42:58), UV detection was performed at 203 nm. The calibration curves showed good linearity over the range of 0.8-12.0 micrograms, gamma = 0.9996-0.9999, the recoveries were 97.1%-100.2% for the nine steroidal saponins. The method is simple, fast, sensitive and accurate and has been applied to the analysis of Rhizoma paridis. The results showed that the contents of the nine steroidal saponins in Rhizoma paridis were different with different sources.