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Human colorectal malignancy and oral UFT.

Shinobu Sakamoto¹, Kunio Tsukada, Tetsuro Sagara

¹Medical Research Institute, School of Medicine, Tokyo Medical and Dental University, Japan. motoend@tmd.ac.jp

Anticancer Research

|May 23, 2002

Summary

Serum thymidine kinase activity is elevated in advanced colorectal cancer, particularly with metastasis. Well-differentiated tumors show higher activity, and UFT chemotherapy effectively suppresses thymidylate synthase in tumors.

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Area of Science:

Biochemistry
Oncology
Molecular Biology

Background:

Thymidylate synthase (TS) and thymidine kinase (TK) are crucial for pyrimidine nucleotide synthesis.
Elevated TS and TK activities are observed in proliferating tissues, including cancers.

Purpose of the Study:

To investigate serum TK activity in colorectal cancer patients.
To correlate TK activity with clinicopathological features and treatment response.

Main Methods:

Enzyme activity assays for TS and TK.
Analysis of serum TK levels in relation to cancer stage, recurrence, metastasis, and differentiation.
Evaluation of UFT chemotherapy's effect on TS activity in tumorous and non-tumorous tissues.

Main Results:

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Serum TK activities were elevated in late-stage colorectal cancer, including cases with recurrence and distant metastasis (liver, lung, bone).
Well-differentiated colorectal cancer exhibited higher TK activity than moderately-well-differentiated types.
Neoadjuvant UFT chemotherapy showed greater suppression of TS activity in tumorous tissues compared to non-tumorous mucosa.

Conclusions:

Serum TK activity serves as a potential biomarker for advanced colorectal cancer and metastasis.
TK activity correlates with tumor differentiation.
UFT demonstrates effective suppression of TS in colorectal tumors, suggesting therapeutic potential.