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Quantitative determination for cytotoxic Friedo-nor-oleanane derivatives from five morphological types of Maytenus
Waldemar Buffa Filho1, Joaquim Corsino, da Silva Vanderlan Bolzani
1Núcleo de Bioensaio, Biossíntese e Ecofisiologia de Produtos Naturais, Instituto de Química, Universidade Estadual Paulista, CP. 355, 14801-970, Araraquara-SP, Brazil.
Abstract:
Five different morphological types of Maytenus ilicifolia of the same age and harvested under the same conditions showed distinct accumulations of some friedo-nor-oleananes. A rapid, sensitive and reliable reverse-phase HPLC method (employing an external standard) was used for the determination of the cytotoxic triterpenoids, 20 alpha-hydroxymaytenin, 22 beta-hydroxymaytenin, maytenin, celastrol and pristimerin in each of the five types. Well resolved peaks with good detection response and linearity in the range 1.0-100 micrograms/mL were obtained.